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Relationship between cycle threshold value and PFU estimated from eastern equine encephalitis virus–infected mosquito pools, Connecticut, USA, 2009. Mosquito pools negative for virus by plaque titration were assigned a value of 0, and mosquito pools negative by quantitative reverse transcription–PCR were assigned a value of 50. Limit of detection by plaque titration (0.8 log10 PFU/mL) is indicated by the dashed vertical line.
