Table 4.
Comparison of IFN-γ ELISPOT assay and TST for patients with PTB
| IFN-γ ELISPOT assay and result | No. (%) of samples with TST resulta |
P value | |||||
|---|---|---|---|---|---|---|---|
| Negative (0 mm) | Positive |
Total | |||||
| + (5–9 mm) | ++ (10–19 mm) | +++ (≥20 mm) | All positive samples | ||||
| E6 | |||||||
| Positive | 4 (5.71) | 3 (4.29) | 21 (30.00) | 15 (21.43) | 39 (55.71) | 43 (61.43) | 2.04 × 10−4 |
| Negative | 2 (2.86) | 2 (2.85) | 15 (21.43) | 8 (11.43) | 25 (35.71) | 27 (38.57) | |
| Total | 6 (8.57) | 5 (7.14) | 36 (51.43) | 23 (32.86) | 64 (91.43) | 70 | |
| E7 | |||||||
| Positive | 4 (4.00) | 2 (2.00) | 38 (38.00) | 20 (20.00) | 60 (60.00) | 64 (64.00) | 2.55 × 10−6 |
| Negative | 2 (2.00) | 2 (2.00) | 15 (15.00) | 17 (17.00) | 34 (34.00) | 36 (36.00) | |
| Total | 6 (6.00) | 4 (4.00) | 53 (53.00) | 37 (37.00) | 94 (94.00) | 100 | |
| C14 | |||||||
| Positive | 4 (5.88) | 2 (2.94) | 20 (29.41) | 11 (16.18) | 33 (48.53) | 37 (54.41) | 2.94 × 10−4 |
| Negative | 2 (2.94) | 3 (4.41) | 15 (22.06) | 11 (16.18) | 29 (42.65) | 31 (45.59) | |
| Total | 6 (8.82) | 5 (7.5) | 35 (51.47) | 22 (32.36) | 62 (91.18) | 68 | |
| E6+E7 | |||||||
| Positive | 6 (7.06) | 5 (5.88) | 37 (43.53) | 18 (21.18) | 60 (70.59) | 66 (77.65) | 0.02 |
| Negative | 1 (1.18) | 0 (0.00) | 10 (11.76) | 8 (9.41) | 18 (21.18) | 19 (22.35) | |
| Total | 7 (8.24) | 5 (5.88) | 47 (55.29) | 26 (30.59) | 78 (91.76) | 85 | |
| E6+E7+C14 | |||||||
| Positive | 6 (9.83) | 4 (6.56) | 35 (57.38) | 12 (19.67) | 51 (83.61) | 57 (93.44) | 0.75 |
| Negative | 0 (0.00) | 0 (0.00) | 2 (3.28) | 2 (3.28) | 4 (6.56) | 4 (6.56) | |
| Total | 6 (9.83) | 4 (6.56) | 37 (60.66) | 14 (22.95) | 55 (90.16) | 61 | |
a
No individual was found with a TST induration of 1 to 4 mm. The two peptide mixtures, particularly E6+E7+C14, had a higher detection sensitivity than the three single peptides, with a similar sensitivity to that of TST (93.44% and 90.16% for the E6+E7+C14 IFN-γ ELISPOT assay and TST, respectively; P > 0.05). Some level of positive detection was achieved by all IFN-γ ELISPOT assays for both the positive and negative TST detection groups.