Table 1.

Summary statistics for three peptide-KLH conjugates^a

Statistic	FT03		FT04		FT05
	Fresh WB	DBS	Fresh WB	DBS	Fresh WB	DBS
95th percentile	5,262	14,664	14,480	10,697	26,676	18,981
Median intensity	1,066	1,078	5,226	3,513	6,250	3,289
Blank featureb	264	611	1,889	1,279	2,369	1,369
Dynamic rangec	19.93	24.00	7.67	8.36	11.26	9.68
Immunogen rankd	26 (100)	10 (100)	2 (100)	2 (100)	41 (100)	38 (100)

^aMicroarray summary statistics were calculated using GeneSpring (version 7.3.1) and Microsoft Office Excel 2003 software. Raw fluorescence intensities from the average of triplicate arrays were used. BALB/c mice were immunized with peptide conjugates of peptides also present as features on the array.

^bBlank features are features printed containing only spotting buffer.

^cThe dynamic range is calculated as the ratio of the 95th percentile over the blank feature intensity.

^dImmunogen rank is the feature corresponding to the peptide immunogen, with 1 being the feature with the highest RFI. Data in parentheses indicate the percentile of the feature corresponding to the peptide immunogen.