Table 2.
Statistic | FT03 | FT04 | FT05 |
---|---|---|---|
Spearman correlationb | 0.92 | 0.87 | 0.90 |
Fold changec | |||
Raw | 1.00 ± 0.34 | 1.52 ± 0.46 | 1.99 ± 0.66 |
Normalizedd | 0.99 ± 0.30 | 1.05 ± 0.35 | 1.04 ± 0.42 |
Microarray summary statistics were calculated using GeneSpring (version 7.3.1) and Microsoft Office Excel 2003 software. Raw fluorescence intensities from the average of triplicate arrays were used. BALB/c mice were immunized with peptide conjugates of peptides also present as features on the array.
Spearman correlation considers only the relative rank of two arrays.
The fold change is the ratio of individual features on the fresh sample array to the same feature on the dried-blood spot arrays.
Microarrays were normalized to the per chip median.