Table 3.
Storage time | 25°C |
37.8°C |
||
---|---|---|---|---|
Correlationb | Immunogen rankc | Correlation | Immunogen rank | |
Fresh serum | NAd | 2 | NA | 2 |
Overnight | 0.96 | 2 | 0.95 | 2 |
1 wk | 0.95 | 4 | 0.89 | 2 |
2 wk | 0.91 | 3 | 0.85 | 5 |
Microarray summary statistics were calculated using GeneSpring (version 7.3.1) and Microsoft Office Excel 2003 software. Raw fluorescence intensities from the average of triplicate arrays were used. BALB/c mice were immunized with the FT04-peptide conjugates.
Correlation is the Spearman correlation, which considers only the relative rank of array features.
Immunogen rank is the feature corresponding to the peptide immunogen, with 1 being the feature with the highest raw fluorescence intensity. The top-ranked peptide in this experiment is highly reactive to the detection reagents.
NA, not applicable.