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. 2012 Mar;19(3):352–358. doi: 10.1128/CVI.05667-11

Table 3.

Correlations between fresh blood and dried-blood spot immunosignatures across storage conditionsa

Storage time 25°C
37.8°C
Correlationb Immunogen rankc Correlation Immunogen rank
Fresh serum NAd 2 NA 2
Overnight 0.96 2 0.95 2
1 wk 0.95 4 0.89 2
2 wk 0.91 3 0.85 5
a

Microarray summary statistics were calculated using GeneSpring (version 7.3.1) and Microsoft Office Excel 2003 software. Raw fluorescence intensities from the average of triplicate arrays were used. BALB/c mice were immunized with the FT04-peptide conjugates.

b

Correlation is the Spearman correlation, which considers only the relative rank of array features.

c

Immunogen rank is the feature corresponding to the peptide immunogen, with 1 being the feature with the highest raw fluorescence intensity. The top-ranked peptide in this experiment is highly reactive to the detection reagents.

d

NA, not applicable.