Fig 3.
Overall IP-10 and IFN-γ responses (protein). Whole blood from M. bovis-infected cattle (105 CFU by aerosol) was stimulated with recombinant ESAT-6:CFP10 (rE:C), M. bovis PPD (PPDb), pokeweed mitogen (PWM), or medium alone (no stimulation [ns]) for 18 h. Stimulated plasma was harvested and IP-10/IFN-γ protein levels determined by ELISA. Values represent mean ± SEM for responses over the course of the study (n = 30 [5 animals × 6 time points]). *, P < 0.05; #, P = 0.07 (differs from nonstimulated values).