Fig 4.
Kinetics of IP-10 and IFN-γ responses (protein) to M. bovis PPD or ESAT-6:CFP10. Whole blood from M. bovis-infected cattle (105 CFU by aerosol) was stimulated with M. bovis PPD (PPDb), recombinant ESAT-6:CFP10 (rE:C), or medium alone (no stimulation [NS]) for 18 h. Stimulated plasma was harvested and IP-10/IFN-γ protein levels determined by ELISA (mean ± SEM, n = 5). *, differs (P < 0.05) from nonstimulated values at the respective time points after challenge for both rEC and PPDb responses. +, differs (P < 0.05) from nonstimulated values at the respective time points after challenge for rEC responses only.