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Localization of LruC by cellular fractionation with Triton X-114. Fractions of the detergent phase (outer membrane fraction), aqueous phase (periplasmic fraction), and protoplasmic cylinder (inner membrane plus cytoplasm fraction) were separated by SDS-polyacrylamide gel electrophoresis and analyzed by immunoblotting using rabbit antiserum specific for LruC. As a control for fractionation purity, fractions were also analyzed using rabbit antiserum to LipL32 and LipL31 (data not shown).
