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Construction of recombinant epitope plasmid. (A) The single-stranded DNA oligonucleotides (lanes 1 and 2) were annealed to the double-stranded DNA oligonucleotides (lane 3). Lane M, DNA molecular marker. (B) The double-stranded DNA oligonucleotides were cloned into NcoI- and XhoI-digested pET-32c and characterized by EcoRI digestion.
