Analysis of immune cells recruited to PPs after infection with Y. pseudotuberculosis YPIII or the ifp and invC mutant. About 2 × 108 bacteria (YPIII, YP97, and YP98) were used to infect 10 BALB/c mice per strain. Three days after infection mice were sacrificed, PPs were isolated and homogenized, and the cell suspensions were used for FACS analysis. Values on the y axis indicate the percentage of cells relative to the cells isolated from PPs infected with the wild-type strain YPIII. F4/80+, macrophages; Ly6G+ (1A8 clone), neutrophils; CD11c+, dendritic cells; CD3+, T cells; CD19+, B cells. Asterisks indicate results that differed significantly from those for YPIII: *, P < 0.05; **, P < 0.01.