Fig 2.

E. coli E24377A gene expression profiles in response to biologically relevant environmental signals A column chart showing fold change expression values (qRT-PCR) between ETEC E24377A grown in DMEM media and biologically relevant additives: bile (3% [wt/vol]; black bars), preconditioned media from commensal E. coli isolate HS (white bars), enteropathogenic E. coli isolate E2348/69 (gray bars), and Shigella boydii BS512 (horizontally striped bars). Values are based on fold change calculated from ΔΔC_T values. All values are log₂ transformed. Columns with an asterisk indicate a significant P value of <0.001 as calculated by Student's t test. Error bars represent standard deviations of the results determined with at least 2 biological replicates. The reference is E24377A grown in DMEM alone to an OD₆₀₀ of 0.5. PCM, preconditioned media. The responses differed among the various preconditioned media, indicating a species-specific response, whereas the bile response was similar to that observed with LB media as described for Fig. 1.