Fig 4.
Temporal expression of ETEC virulence factors in bile. A time course column chart of E24377A grown in LB-bile to different growth densities (OD600 = 0.2, 0.5, and 0.8) is shown. Values are based on fold change calculated from ΔΔCT values. Columns with an asterisk indicate a significant P value of <0.001 as calculated by Student's t test. Error bars indicate standard deviations of the results determined with biological replicates. Overall, we observed generally increased gene expression as the cultures reached the stationary phase of growth, suggesting that a quorum-sensing mechanism is involved in global ETEC regulation.