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Congo red binding and detection of EHEC curli by immunoblotting. Overnight cultures of wild-type Salmonella enterica serovar Typhimurium and EHEC 86-24 and the isogenic mutants AGT210 (ΔlpfA1 ΔlpfA2), SJL102 (ΔcsgA), and SJL101 (ΔlpfA1 ΔlpfA2 ΔcsgA) were spotted (6 μl) onto LBNS agar containing 40 μg/ml Congo red and 20 μg/ml Coomassie brilliant blue and incubated for 24 h at 30°C (A) or 37°C (B). The positive control for Congo red binding at 30°C was S. Typhimurium.
