Fig 5.

Adherence of E. coli O157:H7 and isogenic lpfA and/or ΔcsgA mutants to T84 cells. Monolayers of T84 cells were inoculated with 10⁷ bacterial cells from an overnight culture of the wild-type EHEC strain 86-24, AGT210 (ΔlpfA1 ΔlpfA2), SJL102 (ΔcsgA), and SJL101 (ΔlpfA1 ΔlpfA2 ΔcsgA) and incubated at 37°C and 5% CO₂ for 3 h. T84 cell monolayers were lysed, and 10-fold serial dilutions were plated on LB agar to determine numbers of CFU/ml. Percentages of bacteria recovered were calculated as (final number of CFU per ml/initial number of CFU per ml) × 100. The single ΔcsgA mutant and the triple mutant were compared by Student's t test (*, P = 0.04).