Fig 4.

Differential leukocyte counts in BALF from vaccinated and challenged BALB/c mice. Relative percentages of macrophages, lymphocytes, neutrophils, and eosinophils in BALF samples after cytocentrifugation and staining. Cell type was determined based on morphology and staining characteristics, as observed under a light microscope. Data represent the means and standard deviations for 500 total cells. Significance was determined by one-way ANOVA with SNK post hoc multiple pairwise comparison (*, P < 0.05; ***, P < 0.001).