Fig 5.

Cytokine concentration in BALF as determined by Luminex bead assay. BALF supernatants were concentrated 10-fold using Amicon 3k filter devices and then assayed using a Bio-Rad Bioplex system. Sample concentrations were interpolated from a standard curve for each cytokine, and limits of detection (lod) were determined. Sample concentrations and lods were then divided by 10 to account for the concentration factor of the BALF. Dots represent concentrations for individual mice. Significance was determined by one-way ANOVA with SNK post hoc multiple pairwise comparison (*, P < 0.05; **, P < 0.01).