Fig 1.

Characteristics of purified and refolded recombinant DBPII-7.18. Recombinant 7.18 was purified from inclusion bodies under denaturing conditions and refolded by rapid dilution. Refolded and reduced forms of the protein were separated on SDS-PAGE gel. Differential mobility of refolded antigen on gel in the presence (+) and absence (−) of dithiothreitol (DTT) indicates the presence of disulfide bonds, a simple indicator of native conformation in refolded antigen. See Fig. S1 in the supplemental material for characteristics of recombinant proteins from all the alleles.