Fig 4.

Targeting PspA to hFcγRI enhances S. pneumoniae-specific antibody responses in hFcγRI Tg mice. C57BL/6 WT and Tg mice were immunized i.n. with PBS or with 25 μg of anti-hFcγRI-PspA (day 0) and boosted twice (days 14 and 28). On day 42 the serum and BAL fluid were collected, and the S. pneumoniae-specific IgG and IgA antibodies were measured by ELISA, as described in Materials and Methods. Antibody data for this experiment represent the mean of 4 to 6 mice ± standard deviation. The results presented are representative of two independent experiments, each experiment using 4 to 6 mice per group (*, P < 0.1; **, P < 0.05).