Fig 5.

Targeting PspA to hFcγRI induces a mixed Th1/Th2-like cytokine response. (A to D) C57BL/6 WT and Tg mice were immunized i.n. with anti-hFcγRI-PspA or PBS (day 0) and then boosted twice on days 14 and 28. On day 42, mouse sera, and BAL fluids were collected, and the S. pneumoniae-specific IgG2c and IgG1 antibody levels were measured by ELISA, as described in Materials and Methods. Antibody data represent the mean of 4 to 6 mice ± standard deviation. (E) WT and Tg mice were immunized i.n. as described above. Splenocytes were harvested on day 10 after the second boost (day 38) and cultured with fixed bacteria (inactivated S. pneumoniae) for 3 days. Supernatants were obtained, and Th1 or Th2 cytokine concentrations were measured by CBA, as described in Materials and Methods. The results are representative of two independent experiments, with each experiment using 4 to 6 mice per group (*, P < 0.1; **, P < 0.05).