Fig 4.

Leukocyte accumulation and gene expression of CXC chemokines in lungs of BALB/c mice infected with Legionella. (A) Total cell numbers and proportions of different inflammatory cells in the BALF of wild-type and IL-17A/F-deficient (A/F*) mice (BALB/c background) at 1, 2, 4, and 7 days after infection with 10⁵ CFU of L. pneumophila. *, P < 0.05 for a comparison between the neutrophil numbers of Il17a^−/− Il17f^−/− (A/F*) and wild-type (WT) mice. (B) Expression of the chemokines CXCL-1 (KC), CXCL-5 (LIX), CXCL-2 (MIP-2), IL-12p35, IFN-γ, and IL-4 in whole-lung tissue of mice at 48 h after infection with L. pneumophila. Data are expressed as fold increase. *, P < 0.05 for a comparison between Il17a^−/− Il17f^−/− (A/F*) and wild-type (WT) mice. Bars indicate mean ± SE (n = 5/group). Results are representative of three independent experiments.