Fig 1.

Flotillin-1 RNA silencing does not affect C. pneumoniae host cell association. A549 cells were transfected with siRNA directed against flotillin-1 (siFLO-1) or with nontargeting oligonucleotide (Scramble) for 3 days and inoculated with C. pneumoniae. (A) Representative immunoblot of siFLO-1- and Scramble-transfected A549 cells with flotillin-1 (FLO-1) and beta-actin-specific antibodies. (B) Infected cells were collected and Chlamydia genome equivalents were assayed by qPCR. Chlamydia genome expression was normalized to Scramble-treated cells. Error bars denote the standard errors of the means from 3 independent experiments done in triplicate.