Fig 3.

Flotillin-1 localizes to Chlamydia inclusions. A549 cells were inoculated with C. pneumoniae K6 (Cpn K6) or C. trachomatis L2 (Ctr L2), and endogenous flotillin-1 was detected at 72 and 48 hpi, respectively, with an antibody against flotillin-1 and anti-goat Alexa Fluor 647-conjugated secondary antibody. Inclusions were detected with antibodies specific for C. pneumoniae IncA (top; Cpn K6) or for C. trachomatis IncA (bottom; Ctr L2) and anti-rabbit Alexa Fluor 488-conjugated secondary antibody. In the merged image, IncA (INCA) is shown in green and flotillin-1 (FLO1) in red. Bacterial and host DNA were stained with DAPI and are shown in blue. Bar, 10 μm.