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Distribution of immunoglobulin G (IgG) and IgM antibodies to Trypanosoma cruzi in dogs. An enzyme-linked immunosorbent assay was used to detect antibodies in dogs in Tejupilco (C) and Toluca (D) in the State of Mexico. Negative controls are shown in A. Seroanalysis of dogs from Chiapas, a T. cruzi–endemic zone, is shown in B. The quadrants in A indicate the following: 1, IgG positive; 2, IgG and IgM negative; 3, IgM positive; 4, IgG and IgM positive.
