Table 1. Greene MassTag panel VHF version 1.0*.
| Target | MassTag | Name | Sequence | Gene |
|---|---|---|---|---|
| ZEBOV | 718 (fwd) | EboZA-U234 | AACACCGGGTCTTAATTCTTATATCAA | L |
| 646 (rev) | EboZA-L319 | GGTGGTAAAATTCCCATAGTAGTTCTTT | ||
| SEBOV | 503 (fwd) | EboSU-U416 | CGAGCCTAACGTTTTGGGC | L |
| 630 (rev) | EboSU-L489 | GCTCCAGGAATTGTTCGGGTA | ||
| MARV | 654 (fwd) | MARV-U12816C | CCCTCCATATCTTAGACAACATATTGTG | L |
| 395 (rev) | MARV-L12994 | CCCAACACTCCTGGTTCACAGC | ||
| LASV† | 558 (fwd) | Las4-U92 | ACTGCATTYTCATACTTYCTRGAATC | NP |
| 686 (rev) | Las4-L257 | CCRGGYTTGACCAGTGCTGT | ||
| RVFV | 658 (fwd) | RVF-U578 | GGATTGACCTGTGCCTGTTGC | N |
| 495 (rev) | RVF-L660 | GCATTAGAAATGTCCTCTTTTGCTGC | ||
| CCHFV | 499 (fwd) | CCHV-U4 | AGAAACACGTGCCGCTTACGCCCA | N |
| 710 (rev) | CCHV-L120 | CCATTTCCYTTYTTRAACTCYTCAAACCA | ||
| HNTV | 479 (fwd) | HAN-U179 | AYACAGCAGCAGTTAGCCTCCT | N |
| 702 (rev) | HAN-L245 | GCT GCC GTA RGT AGT CCC TGTT | ||
| SEOV | 455 (fwd) | SEO-U243 | CAGGATTGCAGCAGGGAAGA | N |
| 602 (rev) | SEOUL-L309 | ATGATCACCAGGYTCTACCCC | ||
| YFV | 467 (fwd) | YF-U186 | GCTGGGAGCGCGGTATC | NS5 |
| 670 (rev) | YF-L249 | GGAAGCCCAATGGTCCTCAT | ||
| KFDV | 483 (fwd) | KYF-U170 | TGGAAGCCTGGCTGAAAGAG | NS5 |
| 614 (rev) | KYF-L233 | TCATCCCCACTGACCAGCAT |
*ZEBOV, Ebola Zaire virus; SEBOV, Ebola Sudan virus; MARV, Marburg virus; LASV, Lassa virus; RVFV, Rift Valley fever virus; CCHV, Crimean-Congo hemorrhagic fever virus; HNTV, Hantaan virus; SEOV, Seoul virus; YFV, yellow fever virus; KFDV, Kyasanur Forest disease virus; fwd, forward; rev, reverse. †Primers were designed on Lassa lineage IV sequences (15) and the recently identified outlier sequence Lassa AV (AF256121).