Table 1. Four overlapping regions amplified by reverse transcription–PCR for full-length genome sequence determination.
Region* | Primer name† | Primer position | Nucleotide sequence of the primers (5´–3´) |
---|---|---|---|
R0 (889–1289) | 889s | 889–911 | CATGCCGACCCGAAGAGGAAAG |
1289as | 1289–1265 | GAAGGAAAGGCCCTCGAGAACAAGA | |
R´1 (305–1161) | 305s | 305–328 | CTCCAGAGGACCCCTTCAGCGAAC |
1161as | 1161–1138 | CCCGCGGGTTGGGGATGTGAACCC | |
R´2 (962–331) | 962s | 962–984 | GTACACTCGAGGAGTGGAAGGCG |
331as | 331–311 | TCTGTTCGCTGAAGGGGTCCT | |
R´3 (120–619) | 120s | 120–140 | GTCCCAAGAGGGCGAGGGGAG |
620as | 619–600 | TCCTGGAGCCGGCAGTCCGG |
*Name of the amplified region and position on the genome (according to Wang et al. [10]). †s, forward primer; as, reverse primer.