Fig 3.

Mid-exponential-phase rocG mutant cells exhibit growth arrest in the presence of CEF. (A) Effect of CEF on the growth of the rocG gudB mutant. CEF (50 ng/ml) was added at the beginning of the culture. Liquid growth assays were performed in LB medium using a Bioscreen C growth analyzer. To determine CFU/ml, viable cell counts were estimated by plating diluted cultures on LB agar plates. Results from three independent experiments were averaged, and the standard deviation is indicated by error bars. (B) Effects of different culture media on the CEF-induced growth arrest of the rocG gudB mutant. Strains were grown in 2× LB, LB supplemented with 2% glucose, and glucose (2%) minimal medium in the presence or absence of CEF. Data are representative of at least three independent experiments.