Fig 7.

yuaFG overexpression by IPTG induction rescues the CEF-sensitive phenotype. (A) Induction of yuaFG expression by IPTG (0.1 mM) restores CEF resistance in the yuaFG mutant and the sigW mutant. Three independent experiments were performed for each strain, and the standard deviation is indicated by error bars. (B) IPTG-dependent induction of yuaFG prevents the growth arrest of rocG mutant cells. The maximal effect was observed at a final concentration of 1 mM IPTG. Liquid growth assays were performed in LB medium using a Bioscreen C growth analyzer. A representative data set is shown.