Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or referencea |
|---|---|---|
| Strains | ||
| W168 | trpC2 | BGSC 1A1 |
| HB13528 | W168 rocG::spc | LFH PCR with W168 |
| HB13541 | W168 rocG::spc gudB::kan | HB13543 chromosomal DNA with HB13528 |
| HB13542 | W168 rocG::spc gudB+ | Gain-of-function mutation in gudB |
| HB13545 | W168 rocG::spc gudB::kan amyE::Pspac(hy)-rocG (cat) | pYH001 with HB13541 |
| HB13543 | W168 gudB::kan | LFH PCR with W168 |
| HB6156 | CU1065 yuaFGI::kan | 6 |
| HB13159 | W168 yuaFGI::kan | HB6156 chromosomal DNA with W168 |
| HB13547 | W168 yuaFG::mls | LFH PCR with W168 |
| HB13548 | W168 yuaGI::mls | LFH PCR with W168 |
| HB13568 | W168 yuaI::mls | LFH PCR with W168 |
| HB5331 | CU1065 yqeZ-yqfAB::kan | 6 |
| HB13566 | W168 yqeZ-yqfAB::kan | HB5331 chromosomal DNA with W168 |
| HB13567 | W168 yqeZ-yqfAB::kan yuaFG::mls | HB5331 chromosomal DNA with HB13547 |
| HB10102 | 168 sigW::mls | 27 |
| HB13549 | W168 sigW::mls | HB10102 chromosomal DNA with W168 |
| HB6208 | W168 sigW::spc | 6 |
| HB13558 | W168 sigW::spc yuaFG::mls | HB6208 chromosomal DNA with HB13547 |
| HB13557 | W168 sigW::mls rocG::spc gudB::kan | HB13549 chromosomal DNA with HB13541 |
| HB13571 | W168 yuaFG::mls amyE::Pspac(hy)-yuaFG (cat) | pYH002 with HB13547 |
| HB13572 | W168 rocG::spc gudB::kan amyE::Pspac(hy)-yuaFG | pYH002 with HB13541 |
| HB13574 | W168 sigW::mls amyE::Pspac(hy)-yuaFG (cat) | pYH002 with HB13549 |
| HB13042 | W168 amyE::Pxyl-sigW (cat) | 21 |
| HB13122 | W168 PfabHAFfabHA(P5*)-fabF amyE::Pxyl-sigW (cat) | 21 |
| HB13160 | W168 yuaFGI::kan amyE::Pxyl-sigW (cat) | HB6156 chromosomal DNA with HB13042 |
| HB13226 | W168 PfabHAFfabHA(P5*)fabF yuaFGI::kan amyE::Pxyl-sigW (cat) | HB6156 chromosomal DNA with HB13122 |
| HB13236 | W168 yqeZ-yqfAB::kan amyE::Pxyl-sigW (cat) | HB13566 chromosomal DNA with HB13042 |
| Plasmids | ||
| pPL82 | IPTG-inducible expression vector (amyE integration) | 33 |
| pYH001 | Pspac(hy)-rocG in pPL82 | This study |
| pYH002 | Pspac(hy)-yuaFG in pPL82 | This study |