Fig 5.

Elution profile of the CheA/CheS complex using size exclusion chromatography on two serial Superdex 200 (5/15) columns coupled with inline multiangle light scattering. Solid and dashed lines, respectively, are relative refractive index (RI) and relative UV absorbance at 280 nm. The peak at 6.85 min is a high-molecular-weight aggregate. Apparent molecular masses derived from the light scattering data (filled triangles and filled squares) suggest complexes with ∼180 kDa and ∼100 kDa for the peak at 8.75 min and 10.94 min, respectively. For comparison, the retention times of bovine serum albumin monomer (66.5 kDa) and dimer (133 kDa) are indicated by vertical lines.