Fig 3.

Expression of gudB gene and GudB protein level. (A) Northern blot analysis was performed to determine the stability of the gudB mRNA of B. subtilis GP747 and the isogenic gudB1 mutant GP753. Both mRNAs do not differ in stability and half-life, implying that the direct repeat does not influence mRNA levels. (B) A Western blot analysis was performed to compare the protein levels of GudB with the level of GudB1. Crude extracts of B. subtilis GP747 and the isogenic gudB1 mutant GP753 were used, and the GudB protein was detected by using antibodies raised against RocG that cross-react with the GudB protein.