Table 1.
B. subtilis strains used in this study
| Strain | Genotype | Source or referencea |
|---|---|---|
| 168 | trpC2 | Laboratory collection |
| BG427 | trpC2 metB5 amyE sigB xin-1 attSP recU::cat | 20 |
| BP12 | trpC2 ΔgudB::aphA3 rocG::Tn10 spc amyE::(gudB1mut1 lacZ cat) | Spontaneous mutation of GP1179 on SP |
| BP13 | trpC2 ΔgudB::aphA3 rocG::Tn10 spc amyE::(gudB1mut3 lacZ cat) | Spontaneous mutation of GP1197 on SP |
| GP747 | trpC2 rocG::Tn10 spc | 17 |
| GP753 | trpC2 rocG::Tn10 spc gudB1 | Spontaneous mutation of GP747 on SP |
| GP754 | trpC2 rocG::cat amyE::(gltA-lacZ aphA3) | 16 |
| GP804 | trpC2 gudB1 amyE::(gltA-lacZ aphA3) | 19 |
| GP891 | trpC2 recU::cat | BG427→168 |
| GP892 | trpC2 rocG::spc recU::cat | BG427→GP747 |
| GP894 | trpC2 ΔsbcDC::aphA3 | See Materials and Methods |
| GP895 | trpC2 ΔrecJ::aphA3 | See Materials and Methods |
| GP896 | trpC2 rocG::spc ΔsbcDC::aphA3 | GP747→GP894 |
| GP897 | trpC2 rocG::spc ΔrecJ::aphA3 | GP747→GP895 |
| GP898 | trpC2 ΔexoA::aphA3 | See Materials and Methods |
| GP900 | trpC2 rocG::spc ΔexoA::aphA3 | GP747→GP898 |
| GP1101 | trpC2 amyE::(gudB-lacZ cat) | pGP651→168 |
| GP1102 | trpC2 gudB1 amyE::(gudB-lacZ cat) | pGP651→GP804 |
| GP1103 | trpC2 rocG::Tn10 spc recA::erm cat | IRN444→GP747 |
| GP1104 | trpC2 rocG::Tn10 spc amyE::(gudB-lacZ cat) | pGP651→GP747 |
| GP1105 | trpC2 rocG::Tn10 spc amyE::(gudB-lacZ cat) gudB1 | Spontaneous mutation of GP1104 on SP |
| GP1106 | trpC2 ΔaddAB::spc | HVS666→168 |
| GP1107 | trpC2 ΔaddAB::spc rocG::cat amyE::(gltA-lacZ aphA3) | GP1106→GP754 |
| GP1123 | trpC amyE::(alf-aphA3 lacZ cat) | pGP655→168 |
| GP1127 | trpC2 amyE::(alf1-aphA3 lacZ cat) | Spontaneous mutation of GP1123 on SP-Km |
| GP1160 | trpC2 ΔgudB::aphA3 | See Materials and Methods |
| GP1161 | trpC2 ΔgudB::aphA3 rocG::Tn10 spc | GP1160→GP747 |
| GP1163 | trpC2 ΔgudB::aphA3 rocG::Tn10 spc amyE::(gudB lacZ cat) | pGP900→GP1161 |
| GP1167 | trpC2 Δmfd::ermC | See Materials and Methods |
| GP1168 | trpC2 Δmfd::ermC amyE::(alf-aphA3 lacZ cat) | GP1167→GP1123 |
| GP1169 | trpC2 rocG::Tn10 spc Δmfd::ermC | GP1167→GP747 |
| GP1175 | trpC2 ΔuvrAB::Ermr | See Materials and Methods |
| GP1176 | trpC2 ΔuvrAB::ErmrrocG::Tn10 spc | GP1175→GP747 |
| GP1177 | trpC2 ΔgudB::aphA3 amyE::(gudBmut1 lacZ cat) | pGP1714→GP1160 |
| GP1178 | trpC2 ΔgudB::aphA3 amyE::(gudBmut2 lacZ cat) | pGP1715→GP1160 |
| GP1179 | trpC2 ΔgudB::aphA3 rocG::Tn10 spc amyE::(gudBmut1 lacZ cat) | GP747→GP1177 |
| GP1180 | trpC2 ΔgudB::aphA3 rocG::Tn10 spc amyE::(gudBmut2 lacZ cat) | GP747→GP1178 |
| GP1190 | trpC2 ΔmutSL::aphA3 | See Materials and Methods |
| GP1191 | trpC2 ΔmutSL::aphA3 rocG::Tn10 spc | GP747→GP1190 |
| GP1192 | trpC2 ΔmutSL::aphA3 ΔuvrAB::ErmrrocG::Tn10 spc | GP1176→GP1190 |
| GP1197 | trpC2 ΔgudB::aphA3 rocG::Tn10 spc amyE::(gudBmut3 lacZ cat) | pGP1721→GP1161 |
| GP1198 | trpC2 ΔgudB::aphA3 rocG::Tn10 spc amyE::(gudB1mut3 lacZ cat) | Spontaneous mutation of GP1197 on SP |
| GP1502 | trpC2 Δnfo::cat | See Materials and Methods |
| GP1503 | trpC2 ΔexoA::aphA3 Δnfo::cat | GP1502→GP898 |
| GP1504 | trpC2 ΔexoA::aphA3 Δnfo::cat rocG::spc | GP747→GP1503 |
| HVS666 | trpC2 ΔaddAB::spc | 14 |
| IRN444 | trpC2 recA::erm cat | 34 |
a
Arrows indicate construction by transformation.