Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| E. coli K-12 strains | ||
| MG1655 | F− λ−ilvG rfb-50 rph-1 | Laboratory collection |
| ΔrelBE strain | MG1655 ΔrelBE | This study |
| ΔrhaBAD strain | MG1655 ΔrhaBAD | This study |
| IKA119 | MG1655 ΔrhaBAD ΔrelBE | This study |
| IKA121 | MG1655 ΔrhaBAD ΔrelBE PrhaB-relE | This study |
| IKA121 ΔtnaA | IKA121 ΔtnaA::Kmr | This study |
| Plasmids | ||
| pKD13 | Kmr; template for mutant construction | 12 |
| pKD46 | Ampr; temperature-sensitive λ red recombinase expression vector | 12 |
| pCP20 | Ampr Cmr; temperature-sensitive replication and thermal induction of FLP recombinase | 7 |
| pBAD18 | Ampr; arabinose-inducible expression plasmid | 17 |
| pCA24N | Cmr; lacIq pCA24N | 26 |
| pCA24N-relB | Cmr; lacIq pCA24N PT5-lac::relB+ | 26 |