Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa PA14 | ||
| WT | Clinical isolate UCBPP-PA14 | 38 |
| ΔbqsR mutant | Deletion in bqsR | This study |
| ΔbqsR mutant and pMQ64 | Deletion in bqsR; contains plasmid pMQ64 | This study |
| ΔbqsR mutant and pMQ64-bqsR | Deletion in bqsR; contains plasmid pMQ64-bqsR | This study |
| ΔbqsS mutant | Deletion in bqsS | This study |
| feoB mutant | feoB::MAR2xT7; transposon mutant containing Gmr cassette | 26 |
| E. coli | ||
| UQ950 | E. coli DH5α λ(pir) host for cloning; F-Δ(argF-lac)169 θ80 dlacZ58(ΔM15) glnV44(AS) rfbD1 gyrA96(Nalr) recA1 endA1 spoT1 thi-1 hsdR17 deoR λpir+ | D. Lies, Caltech |
| BW29427 | Donor strain for conjugation; thrB1004 pro thi rpsL hsdS lacZ ΔM15RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)] | W. Metcalf, University of Illinois |
| S. cerevisiae | ||
| InvSc1 | MATa/MATα leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3-Δ1/his3-Δ1 | Invitrogen |
| Plasmids | ||
| pMQ30 | pEX18-Gmr, URA3, CEN6, ARSH4; allelic replacement vector | 42 |
| pΔbqsR | 2-kb fusion PCR fragment containing the ΔbqsR mutant cloned into the BamHI/EcoRI site of pMQ30; used to make the ΔbqsR mutant | This study |
| pΔbqsS | 2-kb fusion PCR fragment containing the ΔbqsS mutant cloned into the BamHI/EcoRI site of pMQ30; used to make the ΔbqsS mutant | This study |
| pMQ64 | pMQ30 with a deletion of SacB | 42 |
| pMQ64-bqsR | bqsR cloned into pMQ64 | This study |
a
Gmr, gentamicin resistant; Nalr, nalidixic acid resistant.