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Two-dimensional gel analysis of B. glumae cell extracts. (A) BGR1 (wild type). (B) BGS2 (tofI::Ω) (N-octanoyl homoserine lactone [C8-HSL] synthase mutant). (C) BGS2 cells grown in LB medium supplemented with 1 μM C8-HSL. The cultures were harvested in the stationary phase. A total of 100 μg of protein from bacterial cells was separated and stained with silver nitrate. Gels shown are representative of three independent experiments. Spot Q09 was identified as Usp2.
