Table 1.
Strains and plasmids used in this study
| Bacterial strain or plasmid | Genotype or phenotypea | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsd1 hsdR17(rK− mK+) deoR thi-1 supE44 λ−gyrA96 relA1 | Gibco BRL |
| C2110 | polA Nalr | 39 |
| HB101 | F−mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 (Smr) supE44 λ− | Gibco BRL |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| Burkholderia glumae | ||
| BGR1 | Wild type; Rifr | 21 |
| BGS2 | BGR1 tofI::Ω | 25 |
| BGS9 | BGR1 qsmR::Ω | 24 |
| BGQ8/BGSQ8/BGH8 | BGR1 usp1::Tn3-gusA20/BGS2usp1::Tn3-gusA20/BGS9 usp1::Tn3-gusA20 | This study |
| BGQ9/BGSQ9/BGH9 | BGR1 usp2::Tn3-gusA104/BGS2 usp2::Tn3-gusA104/BGS9 usp2::Tn3-gusA104 | This study |
| BGQ10/BGSQ10/BGH10 | BGR1 usp3::Tn3-gusA40/BGS2 usp3::Tn3-gusA40/BGS9 usp3::Tn3-gusA40 | This study |
| BGQ14/BGSQ14/BGH14 | BGR1 usp6::Tn3-gusA242/BGS2 usp6::Tn3-gusA242/BGS9 usp6::Tn3-gusA242 | This study |
| BGQ12/BGSQ12/BGH12 | BGR1 usp7::Tn3-gusA52/BGS2 usp7::Tn3-gusA52/BGS9 usp7::Tn3-gusA52 | This study |
| BGQ16/BGSQ16/BGH16 | BGR1 usp8::Tn3-gusA192/BGS2 usp8::Tn3-gusA192/BGS9 usp8::Tn3-gusA192 | This study |
| BGQ18/BGSQ18/BGH18 | BGR1 usp9::Tn3-gusA136/BGS2 usp9::Tn3-gusA136/BGS9 usp9::Tn3-gusA136 | This study |
| BGQ21/BGSQ21/BGH21 | BGR1 usp10::Tn3-gusA94/BGS2 usp10::Tn3-gusA94/BGS9 usp10::Tn3-gusA94 | This study |
| BGQ22/BGSQ22/BGH22 | BGR1 usp11::Tn3-gusA181/BGS2 usp11::Tn3-gusA181/BGS9 usp11::Tn3-gusA181 | This study |
| BGO6/BGSO6/BGH6 | BGQ8 rpoS::Gmr/BGSQ8 rpoS::Gmr/BGH8 rpoS::Gmr | This study |
| BGO7/BGSO7/BGH7 | BGQ9 rpoS::Gmr/BGSQ9 rpoS::Gmr/BGH9 rpoS::Gmr | This study |
| BGQ82 | BGQ9 usp1::Ω | This study |
| S70/S2S70/S9S70 | BGR1 rpoS::Tn3-gusA70/BGS2 rpoS::Tn3-gusA70/BGS9 rpoS::Tn3-gusA70 | This study |
| Plasmids | ||
| pRK2013 | Tra+; ColE1 replicon; Kmr | 12 |
| pHoKmGus | Promoterless β-glucuronidase gene; Kmr AmprtnpA | 4 |
| pSShe | Cmr | 39 |
| pLysS | Harbors the T7 lysozyme gene; Cmr | Novagen |
| pBluescript II SK(+) | Cloning vehicle, phagemid, pUC derivative; Ampr | Stratagene |
| pLAFR3 | Tra− Mob+ RK2 replicon; Tetr | 40 |
| pRK415 | Mob+lacZ Tetr | 23 |
| pBSGm | 0.8-kb DNA fragment harboring a gentamicin cassette cloned into pBluescriptII SK(+); Ampr Gmr | J.-G. Kim |
| pBGT63 | 2.2-kb DNA fragment harboring qsmR cloned into pLAFR3 | 24 |
| pQSMR-His | qsmR in pET21b; Ampr | 24 |
| pHP45Ω | Ω cassette; Spr Smr | 35 |
| pBGQ8 | 21.5-kb DNA fragment harboring usp1 in pLAFR3 | This study |
| pBGQ9 | 21.5-kb DNA fragment harboring usp2 in pLAFR3 | This study |
| pBGQ11 | 20.1-kb DNA fragment harboring usp7 in pLAFR3 | This study |
| pBGQ12 | 19.4-kb DNA fragment harboring usp3 to usp6 and usp8 to usp11 in pLAFR3 | This study |
| pRPOS | 22.8-kb DNA fragment harboring rpoS in pLAFR3 | This study |
| pRPOS1 | 0.8-kb gentamicin cassette inserted into the AfeI site in rpoS in pRK415 | This study |
| pUSP1 | 2.0-kb cassette inserted into the EcoNI site in usp1 in pRK415 | This study |
| pHS1 | 1,046-bp PCR product harboring usp1 and its promoter region cloned into pRK415 | This study |
| pHS2 | 1,649-bp EcoRI DNA fragment harboring usp2 and its promoter region cloned into pRK415 | This study |
| pRPOS-His | rpoS in pET14b; Ampr | This study |
a
Where more than one strain is listed in a single row, the corresponding phenotypes are separated by slashes. Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Nalr, nalidixic acid resistance; Rifr, rifampin resistance; Smr, streptomycin resistance; Spr, spectinomycin resistance; Tetr, tetracycline resistance; Gmr, gentamicin resistance.