Table 2.
Expression of usp genes in B. glumae
| Tn3-gusA fusion | Mean sp act of β-glucuronidase (10−11 U CFU−1 min−1) ± SDa |
||||
|---|---|---|---|---|---|
| BGR1 (wild type) | BGS2 (tofI::Ω) | BGS9 (qsmR::Ω) | BGS2 + 1μM C8-HSL | BGS9 (pBGT63) | |
| usp1::Tn3-gusA20 | 137.6 ± 10.0 | 34.6 ± 7.2 | 30.7 ± 4.5 | 112.3 ± 5.6 | 76.5 ± 9.2 |
| usp2::Tn3-gusA104 | 364.8 ± 9.8 | 24.0 ± 3.3 | 55.5 ± 7.8 | 185.4 ± 6.4 | 152.0 ± 8.7 |
| usp3::Tn3-gusA40 | 48.4 ± 3.0 | 16.1 ± 0.4 | 17.3 ± 0.6 | 50.8 ± 5.4 | 40.0 ± 2.8 |
| usp6::Tn3-gusA242 | 35.1 ± 1.1 | 9.18 ± 0.2 | 12.4 ± 0.8 | 39.6 ± 2.3 | 25.8 ± 1.7 |
| usp7::Tn3-gusA52 | 35.2 ± 0.9 | 7.0 ± 0.1 | 9.59 ± 0.9 | 34.8 ± 1.4 | 40.4 ± 1.9 |
| usp8::Tn3-gusA192 | 61.9 ± 5.8 | 19.5 ± 0.7 | 12.7 ± 0.8 | 81.7 ± 3.1 | 48.0 ± 2.9 |
| usp9::Tn3-gusA136 | 61.1 ± 0.9 | 22.3 ± 1.1 | 30.0 ± 1.0 | 65.1 ± 4.9 | 50.3 ± 3.7 |
| usp10::Tn3-gusA94 | 54.4 ± 7.2 | 17.1 ± 0.5 | 9.65 ± 0.6 | 65.0 ± 3.7 | 49.2 ± 4.2 |
| usp11::Tn3-gusA181 | 52.1 ± 4.1 | 16.8 ± 0.8 | 12.7 ± 0.8 | 65.5 ± 5.1 | 52.3 ± 4.3 |
a
Bacterial cells were grown for 12 h at 37°C. One unit of β-glucuronidase was defined as 1 nmol 4-methylumbelliferone released per bacterium per min. All values are means ± standard deviations of values from triplicate experiments.