Fig 3.

Effect of GlcNAc on growth of the nagB mutant. (A) Effects of GlcNAc on growth of S. coelicolor A3(2) M145 (■) and the nagB null mutant GAM5 (●). Strains were grown in liquid MM supplemented with either mannitol (50 mM) (M; solid lines), GlcNAc (25 mM) (N; dashed lines), or mannitol plus GlcNAc (M+N; dotted lines). (B) Effects of GlcNAc induction on growth of S. coelicolor A3(2) M145 (■) and the nagB null mutant GAM5 (●). Strains were grown in liquid NMMP supplemented with mannitol (1% [wt/vol]), followed by the addition of 25 mM GlcNAc to the cultures at an OD₆₀₀ of ∼0.2. The addition of GlcNAc is indicated with an arrow. (C) Same as panel A, but with spores that were pregerminated for 8 h in 2× YT at 30°C.