Fig 5.

Phenotypic analysis of nag mutants. Phenotypes of S. coelicolor M145 and its nagA, nagB, nagK, and nagAB mutants (designated GAM4, GAM5, GAM6, and GAM9, respectively) were analyzed by stereomicroscopy. Colonies were grown for 5 days on MM (A) or R5 (B) agar plates with either mannitol (top) or GlcNAc (bottom). For phenotypes of the strains on other media, see Fig. S1 in the supplemental material. Note that nagB mutants failed to grow (NG) on MM supplemented with GlcNAc, while on R5 agar plates with GlcNAc, nagB mutants produced only a very thin layer of biomass. For complementation of the nagA and nagB mutants, see Fig. S3 in the supplemental material.