Table 3.
Maximal growth rates, final OD600 values, substrate consumption rates, and other characteristics of different C. glutamicum strains grown on different carbon sourcesa
| C. glutamicum strain and genotype | Carbon source(s) (concn [%, wt/vol]) | Mean maximal growth rate (h−1) ± SD | Mean final OD600 ± SD | Substrate consumption rate (mmol g CDW−1 h−1) ± SD | Description |
|---|---|---|---|---|---|
| RES167 | Glucose (0.8) | 0.36 ± 0.03 | 13.7 ± 0.3 | 4.5 ± 0.4 | |
| ΔatlR | Glucose (0.8) | 0.35 ± 0.04 | 13.7 ± 1.5 | 4.8 ± 1.4 | |
| ΔrbtT | Glucose (0.8) | 0.39 ± 0.04 | 13.2 ± 0.2 | 4.9 ± 0.9 | |
| ΔatlR-ΔrbtT | Glucose (0.8) | 0.35 ± 0.03 | 12.5 ± 1.8 | 5.0 ± 0.8 | |
| ΔmtlD | Glucose (0.8) | 0.34 ± 0.04 | 13.3 ± 0.1 | 4.5 ± 1.1 | |
| ΔatlR-ΔmtlD | Glucose (0.8) | 0.34 ± 0.01 | 13.1 ± 1.8 | 4.9 ± 1.2 | |
| ΔxylB | Glucose (0.8) | 0.34 ± 0.02 | 13.4 ± 0.8 | 4.0 ± 0.9 | |
| ΔatlR-ΔxylB | Glucose (0.8) | 0.34 ± 0.03 | 12.6 ± 1.1 | 4.2 ± 1.2 | |
| RES167 | Arabitol (0.8) | 0.35 ± 0.03 | 11.5 ± 0.9 | 4.6 ± 0.2 | |
| ΔatlR | Arabitol (0.8) | NG | NG | 3.3 ± 0.3 | |
| ΔrbtT | Arabitol (0.8) | 0.13 ± 0.02 | 7.6 ± 0.7 | 1.9 ± 0.6 | Lag phase of about 4 h |
| ΔatlR-ΔrbtT | Arabitol (0.8) | NG | NG | 2.3 ± 0.5 | |
| ΔmtlD | Arabitol (0.8) | NG | NG | <0.2 | |
| ΔatlR-ΔmtlD | Arabitol (0.8) | NG | NG | <0.2 | |
| ΔxylB | Arabitol (0.8) | NG | NG | 0.4 ± 0.2 | Xylulose formation (up to 3 mM) |
| ΔatlR-ΔxylB | Arabitol (0.8) | NG | NG | 3.2 ± 0.3 | Xylulose formation (up to 9 mM) |
| RES167 | Mannitol (0.8) | NG | NG | 0.4 ± 0.2 | |
| ΔatlR | Mannitol (0.8) | 0.35 ± 0.04 | 11.4 ± 0.5 | 4.7 ± 0.4 | Fructose formation (up to 12 mM) |
| ΔrbtT | Mannitol (0.8) | NG | NG | <0.2 | |
| ΔatlR-ΔrbtT | Mannitol (0.8) | 0.32 ± 0.02 | 11.9 ± 0.5 | 5.3 ± 0.6 | |
| ΔmtlD | Mannitol (0.8) | NG | NG | <0.2 | |
| ΔatlR-ΔmtlD | Mannitol (0.8) | NG | NG | <0.2 | |
| ΔxylB | Mannitol (0.8) | NG | NG | 0.5 ± 0.2 | |
| ΔatlR-ΔxylB | Mannitol (0.8) | 0.35 ± 0.03 | 11.6 ± 0.5 | 4.4 ± 0.5 | Fructose formation (up to 12 mM) |
| RES167 | Arabitol (0.3) + mannitol (0.5) | 0.24 ± 0.01 | 10.2 ± 1.4 | Arabitol, 4.7 ± 0.5; mannitol, 3.1 ± 0.5 | Biphasic growth, first on arabitol and then on mannitol |
| ΔatlR | Arabitol (0.3) + mannitol (0.5) | 0.12 ± 0.01 | 7.0 ± 1.1 | Arabitol, 2.7 ± 0.5; mannitol, 1.4 ± 0.4 | Growth on mannitol only when arabitol was completely consumed |
| ΔxylB | Arabitol (0.3) + mannitol (0.5) | NG | NG | Arabitol, 0.6 ± 0.1; Mannitol, <0.2 | Xylulose formation (up to 1.5 mM) |
| ΔatlR-ΔxylB | Arabitol (0.3) + mannitol (0.5) | NG | NG | Arabitol, 1.8 ± 0.3; mannitol, <0.2 | Xylulose formation (up to 5 mM) |
| RES167 | Glucose (0.3) + mannitol (0.5) | 0.35 ± 0.04 | 4.5 ± 0.4 | Glucose, 5.8 ± 1.0; mannitol, <0.2 | No consumption of mannitol |
| ΔatlR | Glucose (0.3) + mannitol (0.5) | 0.34 ± 0.01 | 11.7 ± 0.4 | Glucose, 1.4 ± 0.2; mannitol, 2.8 ± 0.3 | Parallel consumption of both substrates |
| RES167 | Glucose (0.3) + arabitol (0.5) | 0.36 ± 0.02 | 10.4 ± 0.7 | Glucose, 2.2 ± 0.2; arabitol, 4.9 ± 0.4 | Parallel consumption of both substrates |
| ΔatlR | Glucose (0.3) + arabitol (0.5) | NG | NG | Glucose, <0.2; arabitol, 2.6 ± 0.4 | No consumption of glucose |
| ΔmtlD | Glucose (0.3) + arabitol (0.5) | 0.15 ± 0.01 | 4 ± 0.3 | Glucose, 2.7 ± 0.2; arabitol, <0.2 | No consumption of arabitol and decreased growth rate |
| ΔxylB | Glucose (0.3) + arabitol (0.5) | 0.27 ± 0.04 | 4.2 ± 0.3 | Glucose, 3.7 ± 0.2; arabitol, 1.4 ± 0.4 | Xylulose formation (up to 8.5 mM) |
| ΔatlR-ΔxylB | Glucose (0.3) + arabitol (0.5) | 0.25 ± 0.03 | 4.2 ± 0.2 | Glucose, 3.9 ± 0.5; arabitol, 3.7 ± 1.5 | Xylulose formation (up to 12 mM) |
a
NG, not grown.