Table 1.
C. acetobutylicum strains and plasmids used in this study
| Strain or plasmid | Description | Source or referencea |
|---|---|---|
| C. acetobutylicum strains | ||
| ATCC 824 | Wild type | ATCC |
| araK::intron mutant | araK-inactivated mutant; Emr | This study |
| araR::intron mutant | araR-inactivated mutant; Emr | This study |
| araK::intron pSY9-araK strain | araK-complemented strain; Cmr | This study |
| Plasmids | ||
| pWJ1 | Group II intron | 39 |
| pWJ1-araK | pWJ1 derivative for inserting intron into araK | This study |
| pWJ1-araR | pWJ1 derivative for inserting intron into araR | This study |
| pSY9 | pIMP1-derived vector; Ampr Cmr | 28 |
| pSY9-araK | pSY9 derivative carrying CAC1344 gene | This study |
| pET28a | Novagen | |
| pET28a-araK | pET28a derivative carrying CAC1344 gene | This study |
| pET28a-araR | pET28a derivative carrying CAC1340 gene | This study |
a
ATCC, American Type Culture Collection.