Table 2.
Oligonucleotide primers
| Primer name | Sequence (5′→3′)a |
|---|---|
| FlaAp-F | GAAGCATGCCCTTCAATGCCTTATGC |
| FlaAp-R | GCGAAGCTTGGTCATCGCCGACAC |
| FlrA-R1 | GCTTCCGTGGTCGAGACTTGC |
| FlrA723 | GCCGATGAGCATGCAGGTTAAACT |
| FlrA884 | TCGGGAACACGTTTAAGCGGTAGT |
| FlrA-R2 | GCCCAACAAGGCTACGAAACAG |
| ?>FlrA-R3 | TCCGAATTCACACTGCTCTCCTACA |
| FlrA-F41 | GCCGAACCATAAAAATGATCCGCAA |
| FlrA-R42 | CAGGCTAGTTAGTGGCTTCTATTCT |
| FlrAp-F | GAAGCATGCCTGATCCAAGGTAAG |
| FlrAp-R | GCGAAGCTTAGTGGCTTCTATTCTATT |
| HapA-F29 | GGTGAATGTATTAAGCGTTGAACCG |
| HapA-R259 | GGTAGGTGTCAAATTTTAAAGGCGC |
| HNS-F31 | GATCGCATATGGTAATGTCGGAAATCACTAAG |
| HNS-R32 | GATCGGCTCTTCAGCACAGAGCGAATTCTTCCAGAGA |
| IHFA11 | AAAGGATCCGTGAGCGCCATAAAACTTCC |
| IHFA305 | GAAGGATCCGTCGAAAAATAAGACCGAGC |
| IHFA504 | GCCGAGCTCCGCAGCAGCGGTAGAGG |
| IHFA814 | TAAGCATGCGGCGAGTCTCGTCACCG |
| IHFA-F | GATGCCAAGGATACGGTTGAGGTG |
| IHFA-R | CACGTCGAGCGGTAATAGGAATATC |
| IHFB-F | AGAAAGACTCTGTGCCGAACAAACG |
| IHFB-R | TTCCAACTCCACCTTATCGCCAGTC |
| RecA578 | GTGCTGTGGATGTCATCGTTGTTG |
| RecA863 | CCACCACTTCTTCGCCTTCTTTGA |
| RnnB-F | CGGGGTACCGATTTTCAGCCTGATAC |
| RnnB-R | CGCGGATCCTGGCTTGTAGATATGAC |
| RpoN596 | CCTTTGGTGTGGCATCGCTTAATC |
| RpoN875 | TGACCAGCCATCGACCTTGTTCTT |
| RpoN-F21 | CCCGATAAAAGTCTTCATCCAGCA |
| RpoN-R22 | GAATTCACGCAAGGTCAGGCATCT |
| RpoNp-R | CTAGTCTAGATTGGCCGCCAATTTTGAC |
| RpoNp-F | CGCGGATCCTTTGGGGACATGCAGATC |
| RpoS1020 | CTTCTGCAGTTACTTGTCGTCATCG |
| RpoS-F11 | CCCATATGAGTGTCAGCAATACCGTAACCAA |
| RpoS-R12 | ACGCTCTTCAGCAGTTGTCGTATTCGACGTT |
| RpsM-F51 | GCAACTGCGTGATGGTGTAGCTAA |
| RpsM-R52 | GCTTGATCGGCTTACGCGGACC |
| TcpA-F1 | GTTCATAATTTCGATCTCCACTCCG |
| TcpA-R2 | GTTAACCACACAAAGTCACCTGCAA |
| VC1922-F61 | TAGAAGGTTGACGAAACAAGCAATCA |
| VC1922-R62 | GGTTCAACCACCATAGGTACGAGT |
| VC2520-F | ATGGGCACTTCACTGTTAGGT |
| VC2522-F | GGCGATGCTTTGCTGGATGAAC |
| VC2522-R | CGTTTGAGCCGATAATGTTACCG |
| VC2522-R1 | CACCAATCATCATCGGCAGTTCTCG |
| VC2522-R2 | GGCAATGTTTGAACCGAGGACG |
| VC2522-R3 | AAAGAATTCACCATCATCTCGGGTGCTGAG |
| VC2523-F | ACTGACGGTGATTTACGGCG |
| VC2523-R | CAAGAGTTTACGCCCAAGTGC |
| VC2524-F | CATTACTGGCCGCCGCTCA |
| VC2524-R | TCCAGTTCGTTACGCGCTTG |
| VC2525-F | GGGAAATCACTGCACGCAGAG |
| VC2525-R | CTTTCATCGCTTGACCGTGTG |
| VC2527-F | CGTCGCAACCTTCTCGCAACT |
| VC2527-R | CCCTTTGCCATCACTATCGG |
| VC2528-F | CGCAAACTCTCGGTCGAAGAC |
| VC2528-R | GCCTTGGCTGACAATATACGC |
| VC2529-R | GGTTAGATTCCAATGCTTCCTG |
| VicH5 | GGGAAGCTTGTAATGTCGGAAATCA |
| VicH7 | GTTGCATGCATGTCGGAAATCACTA |
| VicH396 | AGGAGATCTCAGAGCGAATTCTTCC |
| VicH567 | GAGTTCCTGCACCTGCTTTTATATG |
a
Restriction sites used for directional cloning are underlined.