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Response of B. anthracis to excess- or low-iron conditions. The B. anthracis UM23C1-2 wild-type strain (■ and □) and its Δdps2 mutant (▲ and △) were grown to exponential phase (OD540 of 0.3) in LB medium at 37°C and treated with Fe(II)SO4 · 7H2O (1 mM and 2 mM) (A) and the iron chelator DFM (15 mM) (B). The arrows indicate the time points at which the Fe(II)SO4 · 7H2O and DFM were added to the culture. Similar growth kinetics were obtained from a minimum of two independent experiments.
