Fig 3.

Effect of the addition of iron [1 mM Fe(II)SO₄ · 7H₂O)] on the response of wild-type B. anthracis UM23C1-2 and the Δdps2 mutant to paraquat (PQ). Exponentially growing (OD₅₄₀ of 0.3) cultures were pretreated with Fe(II)SO₄ · 7H₂O for 10 min before being challenged with 0.8 mM paraquat. The arrow indicates the time point at which the cultures were pretreated with Fe(II)SO₄ · 7H₂O, followed by the addition of paraquat. Similar growth kinetics were obtained from a minimum of two independent experiments.