Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Trait or relevant genotypea | Source or reference |
|---|---|---|
| Strains | ||
| Bacillus anthracis | ||
| UM23C1-2 | pXO1− pXO2− Ura− Rifr | 17 |
| Δdps2 mutant | UM23C1-2 Δdps2::Kmr | This work |
| ΔperR mutant | UM23C1-2 ΔperR::Kmr | This work |
| Escherichia coli | ||
| TOP10 | F−mcrA (mrr-hsdRMS-mcrBC) ϕ80 lacZΔM15 lacX74 deoR recA1 araD139 (ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen Ltd., Paisley, United Kingdom |
| GM48 | JM110 dam dcm | Stratagene, Amsterdam, Netherlands |
| Plasmids | ||
| pUTE583 | Cmr in E. coli; Ermr in B. anthracis | 7 |
| pUTE618 | Cmr Kmr in E. coli | 7 |
| pGEM-T | Ampr in E. coli | Promega Corp. |
| pHT01 | Ampr Cmr, Pgrac promoter, lacI, E. coli/B. subtilis shuttle vector | MoBiTec, Germany |
| pKG400 | Expression vector for B. anthracis; derivative of pUTE583 containing Pgrac, lacI; Emr Cmr | This work |
| pKG400-dps2 | pKG400 with dps2 cloned downstream of the Pgrac promoter | This work |
a
Abbreviations: Kmr, kanamycin resistance; Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Ermr, erythromycin resistance; Spr, spectinomycin resistance.