Fig 1.

Cytosolic metal profiles of wild-type Bacillus anthracis showing the distribution of the major detectable metal pools. Soluble extracts (50 mg total protein) prepared from wild-type B. anthracis cells were resolved by native 2D-LC (anion exchange followed by size exclusion chromatography) under anaerobic conditions. The resulting fractions were analyzed by ICP-MS for iron (A) and manganese (B) abundances. The z axis represents metal concentration, with color scales set at arbitrary levels. Data represent mean values for three independent biological replicates. The four major iron pools (f1 to f4) and one major manganese pool (m1) are labeled, though note that pools f3 and f4 are partially obscured by the more abundant pool f2 at this resolution (see Fig. 2 and 3).