Fig 6.

Cytosolic metal profiles of wild-type Bacillus anthracis exposed to superoxide stress. Soluble extracts prepared from wild-type B. anthracis cells after 60 min of exposure to PQ were resolved by native 2D-LC (anion-exchange chromatography followed by size exclusion chromatography) under anaerobic conditions. The resulting fractions were analyzed by ICP-MS for iron (A) and manganese (B) abundances. The z axis represents metal concentration, with color scales set at arbitrary levels. Data represent mean values for three independent biological replicates. All scales and labels are as in Fig. 1.