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Iron accumulated by B. anthracis is localized to insoluble, not cytosolic pools. Whole cells (A) and the insoluble (B) and soluble (C) subcellular fractions of cultures used for cytosolic metal profiles (Fig. 1 and 6) were acid digested and analyzed for metal by ICP-MS. Each data set represents the mean of triplicate samples, with error bars representing standard deviations (SD).
