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Immunoblot analysis of Spx content before and after the addition of 1 mM diamide to B. subtilis 1A1 (A) and 1 to 10 mM diamide to S. aureus Newman (B). The relative Spx content as a function of time after no addition (−) or addition (+) of 5 mM diamide to S. aureus Newman is shown in panel C.
