Table 2.
Primers used in this study
| Purpose | Name | Sequence (5′→3′)a |
|---|---|---|
| Fusion PCR and gene deletion | abrB_up_for | GCCGTTTTTCTGTCGTGCGG |
| abrB_up_rev | GGTCCATTCACTATTCTCATTCTCCTCCCAAGAGATACTTA | |
| Km_for | ATGAGAATAGTGAATGGACC | |
| Km_rev | GATTAACAATTATTAGAGGTC | |
| abrB_do_for | GACCTCTAATAATTGTTAATCCCAGCTTCAAAACCTTAAATA | |
| abrB_do_rev | GCGATCGACACGCTGAAATC | |
| rsbV_up_for | AGCAGACGCTTCTCGGAACTA | |
| rsbV_up_rev | ATTGTGAATAGGATGTATTCATTCGTATCACCTCAAATTTTCCT | |
| tet_for | ATGAAATCTAACAATGCGCTCATCGT | |
| tet_rev | TCAGGTCGAGGTGGCCCGGCTCCATG | |
| sigB_do_for | AACATTCTCAAAGGGATTTCTAACCCTCGATGGAGTTAATGTAA | |
| sigB_do_rev | ACAGTTCCAGGTTTTCCCAC | |
| ΔPσB_far_up_for | AGAGTGCTTGAAGCGTATGAA | |
| ΔPσB_up_rev | TAGAAGAACCGCCGCCAGGCAGGAGGTAT | |
| ΔPσB_do_for | CTGGCGGCGGTTCTTCTAATCCTATCAAT | |
| ΔPσB_do_rev | TCTCATTTGATATGCCTCCTCTATTTATTTGCATCATATG | |
| km+SD_for | AGGAGGCATATCAAATGAGAATAGTGAATGGACC | |
| km_rev | GAGGTCATCGTTCAAAATGG | |
| spo0E_do_for | CCATTTTGAACGATGACCTCCGGCCTATCAGATGCATATA | |
| spo0E_do_rev | GAAAAAGGAACCGGTCTCGG | |
| Plasmid cloning | sigB_BamHI_pX1_for | TGGGATCCATGATCATGACACAACCATCA |
| sigB_BamHI_pX1_rev | TGGGATCCTTACATTAACTCCATCGAGGG | |
| Primer extension | spo0E_-400_for | CGGCATGACGATATACAGGA |
| -60b_PσB_rev | ATTACAGATTATACTTTATTT | |
| -7b_spo0E_ingene_rev | TCTTTCTTGTTCAGAAGAACC | |
| Northern blotting | sigB_Nor_for | ATCTGGTTGACATGCTTGCG |
| sigB_T7_rev | CTAATACGACTCACTATAGGGAGAATCGTGACAGTGCTTCCGTC | |
| spo0A_Nor_for | TCCCGATGTGCTCGTATTAG | |
| spo0A_T7_rev | CTAATACGACTCACTATAGGGAGATGGCGGATCGCTCTTTCTAC | |
| spo0E_Nor_for | ATGGGCGGTTCTTCTGAAC | |
| spo0E_T7_rev | CTAATACGACTCACTATAGGGAGATTTATTTGCATCATATGCTGG | |
| spoIIE_Nor_for | GTGCTTGCAGGTGCGCTGAC | |
| spoIIE_T7_rev | CTAATACGACTCACTATAGGGAGACACAACATAACGAGCCAATAT |
a
The underlined portions of the sequences represent T7 promoter sequences.